568 quantitative universal pyrosequencing methylation analysis  (Pyrosequencing Inc)

Journal: BMC cancer

Article Title: RASSF1 tumor suppressor gene in pancreatic ductal adenocarcinoma: correlation of expression, chromosomal status and epigenetic changes.

doi: 10.1186/s12885-016-2048-0

Figure Lengend Snippet: Fig. 3 Methylation status of CpG Islands A and C in PDAC xenografts and cell lines. Panel a shows the mehylation level of 51 CpGs analyzed by pyrosequencing in 14 PDAC xenografts and eight PDAC cell lines. On the left numbers refer to xenografts and cell lines as listed in Additional file 1: Tables S1 and S2. Each of the 51 CpGs, 17 in the promoter and 34 in the first exon, is represented by a square. Numbers on top show the location of CpG dinucleotides and transcription start site is indicated (+1). Any CpG is represented by a square that has one of four grey levels according to the proportion of methylation detected, namely white, light grey, grey and black indicating a level of methylation of 0–20 %, 20–40 %, 40–60 % and >60 %, respectively. Panel b reports the methylation status of the 51 CpGs in the eight indicated PDAC cell lines. Panels c and d report the methylation status of the 37 CpGs whithin the CpG island C in the indicated PDAC xenografts and cell lines, respectively

Article Snippet: Colella S, Shen L, Baggerly KA, Issa JP, Krahe R. Sensitive and quantitative universal Pyrosequencing methylation analysis of CpG sites.

Techniques: Methylation